selection of high affinity dna-aptamer for activated protein c using capillary electrophoresis

Affinity analysis of a protein-aptamer complex using nonequilibrium capillary electrophoresis of equilibrium mixtures.

We propose a new method that allows the use of low-affinity aptamers as affinity probes in quantitative analyses of proteins. The method is based on nonequilibrium capillary electrophoresis of the equilibrium mixture (NECEEM) of a protein with its fluorescently labeled aptamer. In general, NECEEM of a protein with a fluorescently labeled aptamer generates an electropherogram with three characte...

Combining capillary electrophoresis and next-generation sequencing for aptamer selection

Next-generation sequencing (NGS) machines can sequence millions of DNA strands in a single run, such as oligonucleotide (oligo) libraries comprising millions to trillions of discrete oligo sequences. Capillary electrophoresis is an attractive technique to select tight binding oligos or "aptamers" because it requires minimal sample volumes (e.g., 100 nL) and offers a solution-phase selection env...

Affinity Capillary Electrophoresis

Tunable aptamer capillary electrophoresis and its application to protein analysis.

A tunable aptamer electrophoretic assay enables highly sensitive fluorescence detection of multiple proteins and protein isomers. The electrophoretic mobility of proteins is tuned with DNA aptamers binding to the target proteins. Fluorescently labeled aptamers of varying nucleotide lengths serve as both charge modulators for capillary electrophoresis separation and as fluorescent affinity probe...

Selection of a Novel Aptamer Against Vitronectin Using Capillary Electrophoresis and Next Generation Sequencing

Breast cancer (BC) results in ~40,000 deaths each year in the United States and even among survivors treatment of the disease may have devastating consequences, including increased risk for heart disease and cognitive impairment resulting from the toxic effects of chemotherapy. Aptamer-mediated drug delivery can contribute to improved treatment outcomes through the selective delivery of chemoth...

Multiple-Injection Affinity Capillary Electrophoresis

Please address correspondence to Frank A. Gomez at [email protected]. nteractions between biological species are essential to life and are involved directly in many of the enzyme-based reactions involved in cell division, cell death, and cell transformations. These biological interactions are important in the initiation, progression, and harmful effects of human disease including Parkinson...